Database mining and transcriptional analysis of genes encoding inulin-modifying enzymes of Aspergillus niger

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Database mining and transcriptional analysis of genes encoding inulin-modifying enzymes of Aspergillus niger.

As a soil fungus, Aspergillus niger can metabolize a wide variety of carbon sources, employing sets of enzymes able to degrade plant-derived polysaccharides. In this study the genome sequence of A. niger strain CBS 513.88 was surveyed, to analyse the gene/enzyme network involved in utilization of the plant storage polymer inulin, and of sucrose, the substrate for inulin synthesis in plants. In ...

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Prevalence of Genes Encoding Aminoglycoside Modifying Enzymes in Clinical Isolates of Klebsiella Pneumoniae in the Hospitals of Borujerd

Background and Aims: Given the importance of aminoglycoside resistance in nosocomial and community infections caused by bacterial pathogenes such as Klebsiella pneumoniae (K. pneumoniae), the aim of this study was to determine the frequency of aac (6')- Ib and aac (3)- IIa, the genes encoding aminoglycoside modifying enzymes involved in aminoglycoside resistance. Material and Methods: A t...

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The Aspergillus niger multicopper oxidase family: analysis and overexpression of laccase-like encoding genes

BACKGROUND Many filamentous fungal genomes contain complex groups of multicopper oxidase (MCO) coding genes that makes them a good source for new laccases with potential biotechnological interest. A bioinformatics analysis of the Aspergillus niger ATCC 1015 genome resulted in the identification of thirteen MCO genes. Ten of them were cloned and homologously overexpressed. RESULTS A bioinforma...

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Expression of genes encoding cellulolytic enzymes in some Aspergillus species.

Fermentation is an important industrial process for microbial metabolite development and has wide applications in various fields. Aspergillus is the most important genus of fungi used for the production of microbial enzymes such as cellulase. The Aspergillus genome encodes various cellulolytic enzymes. In this study, we assayed the gene expression and cellulolytic enzyme production of three iso...

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Functional analysis of the transcriptional activator XlnR from Aspergillus niger.

The transcriptional activator XlnR from Aspergillus niger is a zinc binuclear cluster transcription factor that belongs to the GAL4 superfamily. Several putative structural domains in XlnR were predicted using database and protein sequence analysis. Thus far, only the functionality of the N-terminal DNA-binding domain has been determined experimentally. Deletion mutants of the xlnR gene were co...

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ژورنال

عنوان ژورنال: Microbiology

سال: 2006

ISSN: 1350-0872,1465-2080

DOI: 10.1099/mic.0.29051-0